Sarcoptes (scabies) and dermatophytes (ringworm) are common in shelter environments and are considered high-risk diseases because of their contagious nature. Ruling out other underlying causes of itching is important to determining a course of action and treatment. This presentation focuses on common causes of itching, diagnostic options, sample collection methods, understanding of results, and therapeutic monitoring. Also included in the presentation is a cost-analysis case study of the 1- to 3-day polymerase chain reaction (PCR) test for Sarcoptes and dermatophytosis versus the traditional ringworm culture.